FAM hydrazide,酰肼绿色荧光染料

FAM hydrazide,酰肼绿色荧光染料
目前Cyanine系列染料以两种异构体的形式存在，一种是非磺化花青素（non-sulfonated cyanines），一种是磺化花青素（sulfonatedcyanine）。两者的光谱性质几乎*，因此大部分应用可相互替换。

FAM hydrazide,酰肼绿色荧光染料

产品介绍

FAM hydrazide, 5-isomer

Many natural and synthetic molecules contain aldehyde or ketone carbonyl groups. These carbonyls react with hydrazides with the formation of hydrazones. The reaction is spontaneous at pH values around neutral, and the resulting hydrazones are very stable.

pounds containing 1,2-diol function, like sugars, can be oxidized with sodium periodate with the formation of carbonyl pounds for the subseuent modification with hydrazides. This is an efficient method for the labeling of glycoproteins (like antibodies), and polysaccharides.

FAM hydrazide is a hydrazide label for the attachment of fluorescein, a bright dye for 488 nm channel, to carbonyl pounds.

General properties

Appearance:	yellow solid
Mass spec M+ increment:	372.07
Molecular weight:	426.81
Molecular formula:	C21H15N2ClO6
Solubility:	good in ethanol, DMF, DMSO
uality cont*ol:	NMR 1H, HPLC-MS (95%)
Storage conditions:	Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Spectral properties

Excitation maximum, nm:	494
ε, L⋅mol−1⋅cm−1:	75000
Emission maximum, nm:	520
Fluorescence uantum yield:	0.9
CF260:	0.20
CF280:	0.17

结构式：

 

FAM hydrazide,酰肼绿色荧光染料

西安凯新生物科技有限公司

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